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negative cd3 enrichment kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc negative cd3 enrichment kit
    (A) qPCR results of Jurkat cells expressing the tools after 6 hours of magnetic induction and stimulation with <t>anti-CD3</t> and anti-CD28 antibodies, compared to normal control cells without expressing the tools. (B) With the employed tools and the induction setup, the induction led to the deactivation of Jurkat cells. However, when <t>anti-CD3</t> and anti-CD28 antibodies were added, the magnetic induction amplified the activation of the cells.
    Negative Cd3 Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/negative cd3 enrichment kit/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    negative cd3 enrichment kit - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Regulating Human T Lymphocytes Through Magnetogenetic Tools"

    Article Title: Regulating Human T Lymphocytes Through Magnetogenetic Tools

    Journal: bioRxiv

    doi: 10.1101/2024.10.08.617204

    (A) qPCR results of Jurkat cells expressing the tools after 6 hours of magnetic induction and stimulation with anti-CD3 and anti-CD28 antibodies, compared to normal control cells without expressing the tools. (B) With the employed tools and the induction setup, the induction led to the deactivation of Jurkat cells. However, when anti-CD3 and anti-CD28 antibodies were added, the magnetic induction amplified the activation of the cells.
    Figure Legend Snippet: (A) qPCR results of Jurkat cells expressing the tools after 6 hours of magnetic induction and stimulation with anti-CD3 and anti-CD28 antibodies, compared to normal control cells without expressing the tools. (B) With the employed tools and the induction setup, the induction led to the deactivation of Jurkat cells. However, when anti-CD3 and anti-CD28 antibodies were added, the magnetic induction amplified the activation of the cells.

    Techniques Used: Expressing, Control, Amplification, Activation Assay

    Expression of CD69 and IL2 genes in T cells after 6 hours of magnetic induction with and without adding anti-CD3 and anti-CD28 antibodies.
    Figure Legend Snippet: Expression of CD69 and IL2 genes in T cells after 6 hours of magnetic induction with and without adding anti-CD3 and anti-CD28 antibodies.

    Techniques Used: Expressing



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    Image Search Results


    (A) qPCR results of Jurkat cells expressing the tools after 6 hours of magnetic induction and stimulation with anti-CD3 and anti-CD28 antibodies, compared to normal control cells without expressing the tools. (B) With the employed tools and the induction setup, the induction led to the deactivation of Jurkat cells. However, when anti-CD3 and anti-CD28 antibodies were added, the magnetic induction amplified the activation of the cells.

    Journal: bioRxiv

    Article Title: Regulating Human T Lymphocytes Through Magnetogenetic Tools

    doi: 10.1101/2024.10.08.617204

    Figure Lengend Snippet: (A) qPCR results of Jurkat cells expressing the tools after 6 hours of magnetic induction and stimulation with anti-CD3 and anti-CD28 antibodies, compared to normal control cells without expressing the tools. (B) With the employed tools and the induction setup, the induction led to the deactivation of Jurkat cells. However, when anti-CD3 and anti-CD28 antibodies were added, the magnetic induction amplified the activation of the cells.

    Article Snippet: To transduce T cells, frozen PBMC samples were thawed, and a negative CD3 enrichment kit (StemCell, 19051) was used to isolate the T cell fraction.

    Techniques: Expressing, Control, Amplification, Activation Assay

    Expression of CD69 and IL2 genes in T cells after 6 hours of magnetic induction with and without adding anti-CD3 and anti-CD28 antibodies.

    Journal: bioRxiv

    Article Title: Regulating Human T Lymphocytes Through Magnetogenetic Tools

    doi: 10.1101/2024.10.08.617204

    Figure Lengend Snippet: Expression of CD69 and IL2 genes in T cells after 6 hours of magnetic induction with and without adding anti-CD3 and anti-CD28 antibodies.

    Article Snippet: To transduce T cells, frozen PBMC samples were thawed, and a negative CD3 enrichment kit (StemCell, 19051) was used to isolate the T cell fraction.

    Techniques: Expressing

    In vitro antitumor function of CAR-T cells. (A, B) The cytotoxic effect of CAR-T cells with different trop2-positive murine tumor cells. The expression of IFN-γ in CD3 + T cells when cocultured with MC38-Trop2 + cells (C), and 4T1-Trop2 + cells (D) were conducted by flow cytometry. For the in vitro phagocytosis assay, BMDMs were cocultured with CFSE-labeled MC38-Trop + cells at a 1:4 ratio in the supernatant of different T cells (E) or the medium of cocultured T cells with tumor cells (F). The cells were harvested after 4 hours coculture, and primary macrophages were identified by flow cytometry using anti-F4/80. Phagocytosis rate was determined as the percentage of CFSE + macrophages. (mean±SEM, n=3). Representative data from at least three independent experiments are shown. *p<0.05, **p<0.01, ****p<0.0001. BMDMs, bone marrow-derived macrophages; CAR, chimeric antigen receptor; CFSE, carboxyfluorescein diacetate succinimidyl ester; ns, not significant; UTD, untransduced.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Delivery of CD47 blocker SIRPα-Fc by CAR-T cells enhances antitumor efficacy

    doi: 10.1136/jitc-2021-003737

    Figure Lengend Snippet: In vitro antitumor function of CAR-T cells. (A, B) The cytotoxic effect of CAR-T cells with different trop2-positive murine tumor cells. The expression of IFN-γ in CD3 + T cells when cocultured with MC38-Trop2 + cells (C), and 4T1-Trop2 + cells (D) were conducted by flow cytometry. For the in vitro phagocytosis assay, BMDMs were cocultured with CFSE-labeled MC38-Trop + cells at a 1:4 ratio in the supernatant of different T cells (E) or the medium of cocultured T cells with tumor cells (F). The cells were harvested after 4 hours coculture, and primary macrophages were identified by flow cytometry using anti-F4/80. Phagocytosis rate was determined as the percentage of CFSE + macrophages. (mean±SEM, n=3). Representative data from at least three independent experiments are shown. *p<0.05, **p<0.01, ****p<0.0001. BMDMs, bone marrow-derived macrophages; CAR, chimeric antigen receptor; CFSE, carboxyfluorescein diacetate succinimidyl ester; ns, not significant; UTD, untransduced.

    Article Snippet: Mouse T cells isolated from wild-type C57BL/6 or BALB/c female mouse splenocytes were purified with a mouse CD3 + T cell negative enrichment kit (BD, USA).

    Techniques: In Vitro, Expressing, Flow Cytometry, Phagocytosis Assay, Labeling, Derivative Assay

    Secretion of SIRPα-Fc by CAR T cells modulate the immune cells in tumor-bearing mouse. Flow cytometry was used to detect the proportion of CAR + in tumor-bearing mouse spleen. (A) CAR positive rate in CD3 + T cells. (B) CAR positive rate in CD4 + T cells, (C) CAR positive rate in CD8 + T cells. (D) CD44 and CD62L of CD3 + CAR + T cells in mouse spleen. Flow cytometry analysis of (E) CD4 + CAR + T cells, (F) CD8 + CAR + T cells, (G) CD45 + CD11c + cells, and (H) CD11b + Ly6G + cells in tumor tissues. (I) Frequency of M1-like (CD206 — MHCII high ) and (J) M2-like (CD206 + MHC II low/neg ) TAMs cell populations in CD45 + CD3 — CD11b + F4/80 + in tumor tissues. All data are presented as mean±SEM from experiments, N=3 mice/group. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CAR, chimeric antigen receptor; MHC, major histocompatibility complex; ns, not significant; SIRPα, signal regulatory protein α; TAM, tumor-associated macrophages; UTD, untransduced.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Delivery of CD47 blocker SIRPα-Fc by CAR-T cells enhances antitumor efficacy

    doi: 10.1136/jitc-2021-003737

    Figure Lengend Snippet: Secretion of SIRPα-Fc by CAR T cells modulate the immune cells in tumor-bearing mouse. Flow cytometry was used to detect the proportion of CAR + in tumor-bearing mouse spleen. (A) CAR positive rate in CD3 + T cells. (B) CAR positive rate in CD4 + T cells, (C) CAR positive rate in CD8 + T cells. (D) CD44 and CD62L of CD3 + CAR + T cells in mouse spleen. Flow cytometry analysis of (E) CD4 + CAR + T cells, (F) CD8 + CAR + T cells, (G) CD45 + CD11c + cells, and (H) CD11b + Ly6G + cells in tumor tissues. (I) Frequency of M1-like (CD206 — MHCII high ) and (J) M2-like (CD206 + MHC II low/neg ) TAMs cell populations in CD45 + CD3 — CD11b + F4/80 + in tumor tissues. All data are presented as mean±SEM from experiments, N=3 mice/group. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CAR, chimeric antigen receptor; MHC, major histocompatibility complex; ns, not significant; SIRPα, signal regulatory protein α; TAM, tumor-associated macrophages; UTD, untransduced.

    Article Snippet: Mouse T cells isolated from wild-type C57BL/6 or BALB/c female mouse splenocytes were purified with a mouse CD3 + T cell negative enrichment kit (BD, USA).

    Techniques: Flow Cytometry